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Relative Sensitivity of Photosynthesis and Respiration to Freeze-Thaw Stress in Herbaceous Species 1: Importance of Realistic Freeze-Thaw Protocols

机译:光合作用和呼吸作用对草本植物冻融胁迫的相对敏感性1:现实冻融方案的重要性

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摘要

The relative effect of a freeze-thaw cycle on photosynthesis, respiration, and ion leakage of potato leaf tissue was examined in two potato species, Solanum acaule Bitt. and Solanum commersonii Dun. Photosynthesis was found to be much more sensitive to freezing stress than was respiration, and demonstrated more than a 60% inhibition before any impairment of respiratory function was observed. Photosynthesis showed a slight to moderate inhibition when only 5 to 10% of the total electrolytes had leaked from the tissue (reversible injury). This was in contrast to respiration which showed no impairment until temperatures at which about 50% ion leakage (irreversible injury) had occurred. The influence of freeze-thaw protocol was further examined in S. acaule and S. commersonii, in order to explore discrepancies in the literature as to the relative sensitivities of photosynthesis and respiration. As bath cooling rates increased from 1°C/hour to about 3 or 6°C/hour, there was a dramatic increase in the level of damage to all measured cellular functions. The initiation of ice formation in deeply supercooled tissue caused even greater damage. As the cooling rates used in stress treatments increased, the differential sensitivity between photosynthesis and respiration nearly disappeared. Examination of agriculturally relevant, climatological data from an 11 year period confirmed that air cooling rates in the freezing range do not exceed 2°C/hour. It was demonstrated, in the studies presented here, that simply increasing the actual cooling rate from 1.0 to 2.9°C/hour, in frozen tissue from paired leaflet halves, meant the difference between cell survival and cell death.
机译:研究了两种马铃薯物种Solanum acaule Bitt中冻融循环对马铃薯叶片组织光合作用,呼吸作用和离子泄漏的相对影响。和茄茄。研究发现,光合作用对冻结压力的敏感性比呼吸作用高得多,并且在观察到任何呼吸功能损害之前,光合作用的抑制作用超过60%。当总电解质中只有5%至10%从组织中泄漏时(可逆性损伤),光合作用显示出轻微至中度的抑制作用。这与呼吸相反,后者在直到发生约50%的离子泄漏(不可逆损伤)的温度下才显示出损害。为了研究光合作用和呼吸作用的相对敏感性方面的文献差异,进一步研究了冻融方案对沙门氏菌和沙门氏菌的影响。随着浴冷却速率从1℃/小时增加到约3或6℃/小时,对所有测得的细胞功能的损害程度显着增加。在深度过冷的组织中结冰的开始甚至造成了更大的破坏。随着用于压力处理的冷却速率增加,光合作用和呼吸之间的差异敏感性几乎消失了。对11年中与农业相关的气候数据进行的检查证实,冷冻范围内的空气冷却速率不超过2°C /小时。在这里提出的研究中,事实证明,将成对的小叶两半的冷冻组织中的实际冷却速率简单地从1.0℃/小时提高到2.9°C /小时,意味着细胞存活与细胞死亡之间的差异。

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